1. What is a Cytiva Flow Rate Calculator?

Definition: This calculator converts linear flow rate (cm/h) to volumetric flow rate (mL/min) for Cytiva chromatography systems.

Purpose: It helps researchers and technicians in bioprocessing determine the appropriate flow rates for chromatography columns.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( Q_{mlpm} \) — Volumetric flow rate (milliliters per minute)
• \( Q_{cmph} \) — Linear flow rate (centimeters per hour)

Explanation: The conversion factor 0.01667 comes from the relationship between linear and volumetric flow rates in chromatography systems.

3. Importance of Flow Rate Calculation

Details: Proper flow rate calculation ensures optimal column performance, resolution, and prevents damage to the chromatography media.

4. Using the Calculator

Tips: Enter the desired linear flow rate in cm/h. The calculator will automatically compute the corresponding volumetric flow rate in mL/min.

5. Frequently Asked Questions (FAQ)

Q1: Why convert between cm/h and mL/min?
A: Chromatography methods often specify linear flow rates (cm/h), while pumps are controlled with volumetric flow rates (mL/min).

Q2: Does this work for all Cytiva columns?
A: Yes, the conversion is universal, but always verify with your specific column's documentation.

Q3: When would I need to adjust flow rates?
A: Flow rates may need adjustment based on column size, media type, or specific protocol requirements.

Q4: How accurate is this conversion?
A: The conversion is mathematically precise, but actual system performance may vary slightly based on equipment.

Q5: Can I use this for non-Cytiva systems?
A: While the principle is similar, other systems may use different conversion factors - consult your manufacturer's documentation.